EK1282

Human Ki-67/MKI67 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:MKI67, Ki-67, MIB-1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.35 pg/mL
  • 检测范围:6.25 pg/mL - 400 pg/mL
  • 回收率:85% - 102%

在售SKU:70-EK1282-48, 70-EK1282-96

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描述

商品名

Human Ki-67/MKI67 ELISA Kit (人KI-67 MKI67 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:1.9% - 2.5%;板间变异系数:2.4%-3.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.35 pg/mL

检测范围

6.25 pg/mL - 400 pg/mL

回收率

85% - 102%

平均回收率

0.95

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Ki-67/MKI67单克隆抗体的96孔聚苯乙烯酶标板
  • 人Ki-67/MKI67冻干标准品
  • 人Ki-67/MKI67检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Ki-67抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Ki-67与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Ki-67的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Ki-67/MKI67 ELISA Kit (人KI-67 MKI67 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 MKI67, Ki-67, MIB-1 靶点信息

MKI67 分子靶点信息概述

  • 分子名:MKI67, marker of proliferation Ki-67
  • 基因家族:Protein phosphatase 1 regulatory subunits
  • 别名:MIB-1; PPP1R105; Ki-67
  • 全称:protein phosphatase 1, regulatory subunit 105; Molecular Immunology Borstel antibody 1; antigen identified by monoclonal antibody Ki-67

MKI67 分子靶点综述

KI-67也称为Ki-67或MKI67,在人类中是一种由MKI67基因编码的蛋白质。Ki-67是350 - 400kDa的核蛋白,属于染色体有丝分裂相关蛋白的成员。此外,Ki-67与核糖体RNA转录有关,其失活会抑制核糖体RNA的合成。Ki-67与160kDa 的Hklp2相互作用,后者促进中心体分离和纺锤体的两极性。它也直接与NIFK相互作用,并结合UBF,从而在核糖体RNA的合成中发挥作用。在ER阳性乳腺癌患者中,Ki-67代表了一群高增殖性的亚群,使他们在辅助化疗中获益更多。

人 Human MKI67 分子靶点信息

  • 分子名:MKI67, marker of proliferation Ki-67
  • 别称:
    • antigen identified by monoclonal antibody Ki-67
    • antigen KI-67
    • antigen Ki67
    • KIA
    • MIB-
    • MIB-1
    • Molecular Immunology Borstel antibody 1
    • PPP1R105
    • proliferation marker protein Ki-67
    • proliferation-related Ki-67 antigen
  • 基因序列:NCBI_Gene: 4288
  • 蛋白序列:UniProtKB: P46013

人 Human MKI67靶点分子功能(预测)

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.

操作步骤


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