EK1302

Human FSH ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:待定
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.36 mIU/ml
  • 检测范围:1.56 mIU/mL - 100 mIU/mL
  • 回收率:82% - 119%

在售SKU:70-EK1302-48, 70-EK1302-96

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描述

商品名

Human FSH ELISA Kit (人促卵泡激素 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.4% - 3.6%;板间变异系数:3.0%-3.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.36 mIU/ml

检测范围

1.56 mIU/mL - 100 mIU/mL

回收率

82% - 119%

平均回收率

0.92

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗FSH单克隆抗体的96孔聚苯乙烯酶标板
  • 人FSH冻干标准品
  • 人FSH检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人FSH抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的FSH与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中FSH的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human FSH ELISA Kit (人促卵泡激素 ELISA试剂盒) - 标准曲线
标准曲线

操作步骤


下载本产品说明书

ELISA操作常见问题

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引用文献


  1. 血清

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