EK2F03

Mouse FGF basic/FGF2/bFGF ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

说明书

分子靶点：FGF2, FGFB
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清
检测样本体积：血清、血浆：10 μL，细胞培养上清：100 μL
灵敏度：27.11 pg/mL
检测范围：46.87 pg/mL - 3000 pg/mL
回收率：85% - 114%

在售SKU:70-EK2F03-48, 70-EK2F03-96, EK2F03

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Size
库存号

描述

商品名	Mouse FGF basic/FGF2/bFGF ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	双抗夹心法
精密度	板内变异系数：8.9% - 9.2%；板间变异系数：2.2% - 6.3%
样本类型	血清，血浆，细胞培养上清
检测样本体积	血清、血浆：10 μL，细胞培养上清：100 μL
灵敏度	27.11 pg/mL
检测范围	46.87 pg/mL - 3000 pg/mL
回收率	85% - 114%
平均回收率	1.01
板式	96孔板，可拆
保存	试剂盒未拆开，2 - 8℃保存。已拆开，标准品-20℃保存，其它2 - 8℃保存。
运输条件	4℃蓝冰运输
组分	酶标板标准品检测抗体标准品稀释液链霉亲和素 10×检测缓冲液显色底物终止液 20×洗液封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长5630nm)测定吸光度值。

分子信息

概述小鼠 Mouse Fgf2 分子靶点信息

FGF2 分子靶点信息概述

分子名：FGF2, fibroblast growth factor 2
基因家族：Receptor ligands; Fibroblast growth factor family
曾用名：FGFB
全称：fibroblast growth factor 2 (basic)

FGF2 分子靶点综述

碱性成纤维细胞生长因子(FGFbasic)，又名bFGF、FGF2或FGF-β，是成纤维细胞生长因子家族中的一员。在正常组织的伤口愈合和肿瘤发展过程中，硫酸乙酰肝素降解酶激活FGFbasic来介导血管生成。另外，人类脂肪细胞可合成和分泌FGFbasic，它的浓度和血液样本中的BMI相关。研究显示低水平的FGFbasic在过度焦虑的发生中发挥重要作用。此外，FGFbasic是人类胚胎干细胞培养基中的重要组成成分，是维持细胞未分化状态的必要因素。FGFbasic与BMP4一起可促进干细胞分化为中胚层谱系。相比于未处理的干细胞，经BMP4和FGFbasic处理过的干细胞通常会分化出更多的成骨细胞和软骨细胞。

分子名：Fgf2, fibroblast growth factor 2
别称：
- bFGF
- Fgf-2
- Fgfb
- fibroblast growth factor, basic
基因序列：NCBI_Gene: 14173
蛋白序列：

小鼠 Mouse Fgf2 靶点分子功能（预测）

Enables growth factor activity. Involved in stem cell development. Acts upstream of or within several processes, including animal organ development; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).