F31FP302

Anti-Rat Foxp3, PE (Clone: FJK-16s) 检测试剂

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¥1,380.00¥2,880.00

  • 分子靶点:FOXP3, IPEX
  • 种属:人 (Human)
  • 标记:PE
  • 亚型:Rat IgG2a, κ
  • 克隆号:FJK-16s

在售SKU, 70-F31FP302-25, 70-F31FP302-100, F31FP302

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描述

商品名

Anti-Rat Foxp3, PE (Clone: FJK-16s) 检测试剂

靶点

FOXP3, IPEX

标记

PE

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Rat IgG2a, κ

克隆号

FJK-16s

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含0.2% BSA和 0.2% ProClin950的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Rat Foxp3, PE (Clone: FJK-16s) 检测试剂 - 结果示例图片
结果示例

图 - 以大鼠血液CD4阳性细胞设门进行分析的结果示例(需要配合使用Transcription Factor Fixation/Permeabilization Buffer),左图为Anti-Rat CD4, APC和Rat IgG2a, PE染色结果,右图为Anti-Rat CD4, APC和Anti-Rat Foxp3, PE染色结果。

分子信息

概述大鼠 FOXP3, IPEX 靶点信息

FOXP3 分子靶点信息概述

  • 分子名:FOXP3, forkhead box P3
  • 基因家族:Forkhead boxes
  • 别名:JM2; XPID; AIID; PIDX; DIETER; SCURFIN
  • 曾用名:IPEX
  • 全称:immune dysregulation, polyendocrinopathy, enteropathy, X-linked

FOXP3 分子靶点综述

FoxP3蛋白是转录因子叉头家族的成员之一,表达于调节性T细胞(Regulatory T Cells, Treg),其发育和功能受到叉头蛋白的影响。T细胞中FoxP3的异常表达会抑制其活性和细胞因子分泌。

大鼠 Rat Foxp3 分子靶点信息

大鼠 Rat Foxp3 靶点分子功能(预测)

Predicted to enable several functions, including DNA-binding transcription repressor activity, RNA polymerase II-specific; NFAT protein binding activity; and enzyme binding activity. Involved in several processes, including establishment of endothelial blood-brain barrier; negative regulation of defense response to virus; and response to rapamycin. Predicted to be located in cytoplasm and nucleoplasm. Predicted to be active in nucleus. Used to study intrahepatic cholangiocarcinoma. Biomarker of Korean hemorrhagic fever; asthma; bronchiolitis obliterans; hypertension; and hypothyroidism. Human ortholog(s) of this gene implicated in Human papillomavirus infectious disease; colorectal cancer; immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome; pulmonary tuberculosis; and type 1 diabetes mellitus. Orthologous to human FOXP3 (forkhead box P3).

操作步骤

文章目录[隐藏]


下载本产品说明书

使用方法

1.收集细胞,调整细胞浓度到106 -107个/ml。
2. 在流式管中加入 50 μl 细胞,加入 表染抗体。涡旋震荡混匀,室温避光孵育 15 分钟。
3. 每管加入 2 ml 1× FCM Lysing Solution 工作液,涡旋震荡混匀,室温避光孵育 15 分钟。室温下 300 - 400 × g 离心 5 分钟,弃去上清。
4. 每管加入 2 ml 1× Flow Cytometry Staining Buffer,涡旋震荡混匀。室温下 300 - 400 × g 离心 5分钟,弃去上清。
5. 每管加入 1 ml 1× Fixation/Permeabilization 工作液,涡旋震荡混匀。
6. 室温避光孵育 30 - 60 分钟。
7. 无需洗涤,每管加入 2 ml 1× Permeabilization Buffer。室温下300 - 400 × g 离心 5 分钟,弃去上清。
8. 用100 μl 1× Permeabilization Buffer重悬沉淀。通常洗涤后残留的液体量就已足够。
9.加入5 μl Anti-Rat Foxp3, PE或5 μl Rat IgG2a Isotype Control, PE。震荡混匀,室温避光孵育至少30分钟。
10.每管加入2 ml 1× Permeabilization Buffer,室温下300 - 400 × g离心5分钟,弃去上清。
11.每管加入 500 μl 1× Flow Cytometry Staining Buffer 重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注金沙6165总站线路检测生物网站或来电咨询。

FCM流式检测常见问题

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