F1102702

Anti-Human CD27, PE (Clone: 09) 检测试剂

¥400.00 – ¥960.00

说明书

分子靶点：CD27, TNFRSF7
种属：人 (Human)
标记：PE
亚型：Mouse IgG1
克隆号：09

在售SKU, 70-F1102702-100, F1102702, 70-F1102702-25

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD27 分子靶点信息概述
CD27 分子靶点综述
- 人 Human CD27 分子靶点信息
- 人 Human CD27靶点分子功能（预测）

商品名	Anti-Human CD27, PE (Clone: 09) 检测试剂
靶点	CD27, TNFRSF7
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1
克隆号	09
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

Anti-Human CD27, PE (Clone: 09) 流式抗体检测试剂 — 结果示例

图 - 人外周血染色左图为Mouse IgG1 Isotype control,PE和Anti-Human CD19,APC，右图为Anti-Human
CD27,PE和Anti-Human CD19,APC的结果。以淋巴细胞设门进行分析。

分子信息

概述人 CD27 靶点信息

CD27 分子靶点信息概述

分子名：CD27, CD27 molecule
基因家族：Tumor necrosis factor receptor superfamily; CD molecules
别名：TNFRSF7
曾用名：S152; Tp55
全称：tumor necrosis factor receptor superfamily, member 7; CD27 antigen; T-cell activation antigen S152

CD27 分子靶点综述

CD27是TNF受体超家族的一员，在幼稚T细胞、记忆B细胞、NK细胞、造血干细胞（HSC）和祖细胞上结构性表达。CD27是一种在CD4+和CD8+T细胞上表达的跨膜磷酸糖蛋白，在T细胞活化时表达增加，并从细胞表面脱落，活化后形成可溶性CD27（sCD27）。

人 Human CD27 分子靶点信息

分子名：CD27, CD27 molecule
别称：
- CD27 antigen
- CD27L receptor
- MGC20393
- S152
- S152. LPFS2
- T cell activation antigen CD27
- T cell activation antigen S152
- T-cell activation antigen CD27
- T14
- TNFRSF7
- Tp55
- tumor necrosis factor receptor superfamily member 7
- tumor necrosis factor receptor superfamily, member 7
基因序列：NCBI_Gene: 939
蛋白序列：UniProtKB: P26842

人 Human CD27靶点分子功能（预测）

Enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process. Involved in extrinsic apoptotic signaling pathway and negative regulation of apoptotic process. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Implicated in lymphoproliferative syndrome 2.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：：取100 μl 抗凝血，加入5 μl Anti-Human CD27, PE 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD27, PE 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。