EK2103

Mouse RAGE ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:AGER, RAGE
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:7.90 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:97% - 125%

在售SKU:70-EK2103-48, 70-EK2103-96

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描述

商品名

Mouse RAGE ELISA Kit (小鼠晚期糖基化终末产物受体 (RAGE) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.0% - 3.4%;板间变异系数:2.2% - 4.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

7.90 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

97% - 125%

平均回收率

1.05

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗RAGE单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠RAGE冻干标准品
  • RAGE检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(22×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠RAGE抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的RAGE与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中RAGE的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse RAGE ELISA Kit (小鼠晚期糖基化终末产物受体 (RAGE) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 AGER, RAGE 靶点信息

AGER 分子靶点信息概述

  • 分子名:AGER, advanced glycosylation end-product specific receptor
  • 基因家族:C2-set domain containing; Scavenger receptors
  • 别名:RAGE; SCARJ1; sRAGE
  • 全称:receptor for advanced glycation end-products

AGER 分子靶点综述

晚期糖基化终末产物受体 (RAGE)是35 kDa的免疫球超家族跨膜受体,其名字来自于与晚期糖基化终末产物结合的能力。鉴于其在先天免疫的炎症功能和通过共同基序识别一类配体的能力,RAGE通常被认为是一类识别受体。RAGE与一些慢性疾病有关,如动脉粥样硬化、末梢血管疾病、心肌梗死、充血性心力衰竭、糖尿病、阿尔茨海默病等。与其它组织相比,RAGE在肺部表达量最高,尤其是在I型肺泡上皮细胞,而在特发性肺纤维化 (IPF)中不表达,这表明RAGE在肺系中的表达和调控与心血管系统中不同。阻断/敲除RAGE会导致细胞黏附受损,增加细胞增殖和迁移。

小鼠 Mouse Ager 分子靶点信息

小鼠 Mouse Ager 靶点分子功能(预测)

Enables S100 protein binding activity; advanced glycation end-product binding activity; and heparin binding activity. Involved in several processes, including cellular response to amyloid-beta; negative regulation of long-term synaptic potentiation; and positive regulation of cytokine production. Acts upstream of or within several processes, including induction of positive chemotaxis; negative regulation of advanced glycation end-product receptor activity; and positive regulation of macromolecule metabolic process. Located in extracellular space and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and lung. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); cystic fibrosis; kidney failure (multiple); and lupus nephritis. Orthologous to human AGER (advanced glycosylation end-product specific receptor).

操作步骤


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