EK1312

Human sICAM-3/CD50 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:ICAM3, ICAM-R, CD50
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:5.09 pg/mL
  • 检测范围:125 pg/mL - 8000 pg/mL
  • 回收率:77% - 112%

在售SKU:70-EK1312-48, 70-EK1312-96

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描述

商品名

Human sICAM-3/CD50 ELISA Kit (人可溶性细胞间粘附分子3 (sICAM-3/CD50) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 3.9%;板间变异系数:1.6% - 6.2%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

5.09 pg/mL

检测范围

125 pg/mL - 8000 pg/mL

回收率

77% - 112%

平均回收率

0.93

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-17AF单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-17AF冻干标准品
  • IL-17AF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sICAM-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的sICAM-3与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sICAM-3的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sICAM-3/CD50 ELISA Kit (人可溶性细胞间粘附分子3 (sICAM-3/CD50) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 ICAM3, ICAM-R, CD50 靶点信息

ICAM3 分子靶点信息概述

  • 分子名:ICAM3, intercellular adhesion molecule 3
  • 基因家族:CD molecules; Immunoglobulin like domain containing; Ig-like cell adhesion molecule family
  • 别名:CDW50; ICAM-R; CD50

ICAM3 分子靶点综述

细胞间粘附分子3 (ICAM-3),也称为CD50,是免疫球蛋白超家族的成员,可与白细胞整合素LFA-1 (CD11a/CD18)和αd/β2结合。ICAM-3在白细胞和表皮朗格汉斯细胞上表达。ICAM-3是一种由白细胞表达的跨膜糖蛋白,是淋巴细胞功能相关抗原(LFA)-1整合素的反受体。ICAM-3除了作为T细胞与抗原呈递细胞接触的粘附分子外,还可调节LFA-1对ICAM-1的亲和力,并诱导T细胞活化和增殖。ICAM-3在T细胞和抗原呈递细胞的免疫应答启动过程中发挥重要作用。CD50分子在T淋巴细胞功能成熟过程中发挥作用,在T淋巴细胞成熟过程中表达增多。

人 Human ICAM3 分子靶点信息

  • 分子名:ICAM3, intercellular adhesion molecule 3
  • 别称:
    • CD50
    • CDW50
    • ICAM-3
    • ICAM-R
    • intercellular adhesion molecule-3
  • 基因序列:NCBI_Gene: 3385
  • 蛋白序列:UniProtKB: P32942

人 Human ICAM3靶点分子功能(预测)

Enables integrin binding activity. Predicted to be involved in cell adhesion. Located in extracellular exosome.

操作步骤


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