Next generation sequencing for miRNA profile of spleen CD4+ T cells in the murine model of acute asthma

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  • 作者：Ye Liu, Zhongqi Chen, Kun Xu, Zhengxia Wang, Chaojie Wu, Zhixiao Sun, Ningfei Ji, Mao Huang, Mingshun Zhang
  • 期刊：Epigenomics
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Aim:To explore the miRNAs profile of CD4+ T lymphocytes in asthma via next generation sequencing.

Methods:In the murine model of acute asthma, spleen CD4+ T lymphocytes were sorted, in which small RNAs were extracted and sequenced. Novel miRNAs were measured with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Results:A total of 127 miRNAs were found to exhibit at least twofold change. In the 262 predicted novel miRNAs, 14 novel miRNAs were measured in qRT-PCR in the sorted CD4+ T cells or in the differentiated Th1/Th2 cells and novel miR-11 (xxx-m0228-3p) was significantly decreased in the sorted CD4+ T cells from the murine model of asthma and in the Th2 cells.

Conclusion:Aberrant miRNAs profile in the CD4+ T lymphocytes from acute asthma was documented.

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