Effect of Methionine on AMD1 Gene Expression in Prostate Cancer Cells

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  • 作者：Shaochen Shen, Linhong Zeng, Hongwei Huang
  • 期刊：NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
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Objective:To investigate the effect of AMD1 gene expression on prostate cancer cells (PC-3M), explore the mechanism of AMD1 action in cancer cells, and examine the regulation of AMD1 gene expression by methionine (MET).

Methods:Quantitative PCR (qPCR) and western blot analysis (WB) approaches were used to detect and measure gene expression. The cell apoptotic rate was determined by flow cytometric (FCM) analysis.

Results:qPCR and WB assays showed that both AMD1 gene expression and cell apoptotic rate were associated with MET.

Conclusion:MET has a significant regulatory effect on the expression of the AMD1 gene and a certain amount of MET can promote the expression of the AMD1 gene. This provides a health guideline for a low-methionine diet for prostate cancer patients and scientific evidence for prostate cancer prevention.

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