Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte

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作者：Fei-Ping Li, Jie-Long Zhou, Ai-Wei Guo, Yu Liu, Fei Zhang, Bai-Hui Xu, Rui Liu, Ya-Long Wang, Ming-Huang Chen, Yan-Hong Lin, Shu-Wen He, Bao-Qiong Liao, Xian-Pei Fu, Hai-Long Wang
期刊：ENVIRONMENTAL POLLUTION
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Di(n-butyl) phthalate (DBP) is extensively used in industrial applications as plasticizer and stabilizer and its presence in the environment may present health risks for human. Previous studies have demonstrated its mutagenic, teratogenic, and carcinogenic ability. However, its effect on mammalian oocyte maturation remains unknown. In this study, we examined the effect of DBP on oocyte maturation both in vitro and in vivo. Our results showed that DBP could significantly reduce mice oocyte germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) rates. In addition, oocyte cytoskeleton was damaged and cortical granule-free domains (CGFDs) were also disrupted. Finally, DBP induced early apoptosis of oocyte and granulosa cells (GCs). Collectively, these data demonstrate that DBP could reduce meiosis competence and mouse oocyte development.