Identification of the immunogenic membrane proteins, Catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori

AimsThis study aims to investigate the specific membrane antigens that are targeted by antibodies raised against H. pylori.Methods and resultsBovine milk antibodies were prepared using whole H. pylori, purified membrane proteins, or both. Enzyme-linked immunosorbent assay (ELISA) and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) experiments revealed that these immunogens triggered anti-H. pylori antibody production in milk. The highest antibody titer was induced by the mixture of whole bacteria and purified membrane proteins. The antibodies induced by mixed immunogens significantly inhibited H. pylori growth in vitro and were used to identify catalase, plasminogen-binding protein A (PgbA), and PgbB via western blotting, immunoprecipitation and two-dimensional western blotting (2D-WB) followed by LC–MS/MS. The immunogenicity of PgbA and PgbB was verified in mice vaccinated with their B-cell epitope vaccines. Following prophylactic vaccination of C57BL/6 mice, each of the three antigens alone and their combination reduced the weight loss in mice, increased antibody titers, and relieved the inflammatory status of the gastric mucosa following H. pylori infection.ConclusionsCatalase, PgbA, and PgbB could serve as valuable membrane antigens for the development of anti-H. pylori immunotherapies.

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