Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides fromSargassum fusiforme. The effects of UV/H2O2treatment on the physicochemical properties and anti-photoaging activity ofS. fusiformepolysaccharides were studied. UV/H2O2treatment effectively degraded polysaccharides fromS. fusiforme(DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function ofS. fusiformepolysaccharides were investigated using human epidermal HaCaT cellsin vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2treatment could effectively improve the anti-photoaging activity ofS. fusiformepolysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.Keywords:anti-photoaging;degradation;HaCaT cells;Sargassum fusiformepolysaccharides;UV/H2O2

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